Arming CRISPR/Cas systems with an enzyme that also controls the translation of genetic information into protein
CRISPR/Cas systems are known as promising “gene scissors” in the genome editing of plants, animals, and microorganisms by targeting specific regions in their DNA – and perhaps they can even be used to correct genetic defects. A team of scientists led by Juliane Behler and Prof. Dr. Wolfgang Hess from the University of Freiburg have now identified an enzyme, a special pair of RNA scissors, which is involved in CRISPR/Cas systems and the correct regulation of gene expression – in other words, in reading genes and translating their information into proteins. The researchers have published their work in the scientific journal Nature Microbiology.
Natural CRISPR/Cas systems can be found in most bacteria and archaea, where they form a microbial immune system with which these organisms can defend themselves against viral intruders. In order for this line of defense to work, a long RNA molecule must first be cut into smaller units. An enzyme is therefore used as a pair of RNA scissors to cut the RNA molecule and “arm” the system. Natural CRISPR/Cas systems often function autonomously: They can be exchanged between different bacteria quickly, and they are not dependent on other proteins in their host cells. An exception to this can be found in the popular CRISPR/Cas9 systems, in which the host enzyme RNase III acts as RNA scissors.
The scientists from the University of Freiburg, who are studying CRISPR/Cas systems, have now demonstrated that the enzyme RNase E acts as RNA scissors in the CRISPR/Cas systems of cyanobacteria. This enzyme is very common, and it can be found not only in photosynthetic cyanobacteria, but also in pathogenic bacteria and plant chloroplasts. It is an important factor for the correct regulation of gene expression within all these groups of organisms. What was not known until now, however, was that it also plays a role in CRISPR/Cas systems.
The team’s research shows that the interaction of CRISPR/Cas systems with the cellular mechanisms of their host organisms is stronger than previously suspected, meaning there could be greater potential for the use of such systems.
Funding for the team’s research came from the Deutsche Forschungsgemeinschaft (German Research Foundation) as part of a grant for the research group Forschergruppe FOR1680: Unravelling the Immune System. Wolfgang Hess is currently a fellow at the Freiburg Institute for Advanced Studies (FRIAS) at the University of Freiburg.
Juliane Behler, Kundan Sharma, Viktoria Reimann, Annegret Wilde, Henning Urlaub, and Wolfgang R. Hess (2018): The host-encoded RNase E endonuclease as the crRNA maturation enzyme in a CRISPR–Cas subtype III-Bv system. Nature Microbiology. doi: 10. 1038/s41564-017-0103-5
Prof. Dr. Wolfgang Hess
Institute of Biology III
University of Freiburg
Phone: +49 (0)761 / 203-2796
Freiburg scientists discover a new pro-survival receptor module on human tumor B cells
Many tumor diseases are caused by an overexpression and deregulation of signaling proteins promoting the prolonged survival and continuous growth of cells. This is also the case for the most frequent hematological tumors in humans namely B cell leukemia or lymphomas also called blood cancer. These tumors often employ the signaling pathways of the B cell antigen receptor (BCR) and its coreceptor CD19 for their extensive growth and metabolic fitness. How exactly these tumors combine BCR and coreceptor signaling was so far unknown.
The research team led by Prof. Dr. Michael Reth and including Dr. Jianying Yang and Dr. Xiaoxui He has now discovered a new pro-survival receptor module on the surface of the human Burkitt lymphoma B cell line Ramos. The scientists have published these findings in the EMBO Journal.
The full article can be found on the Bioss website.
Immunologist is new member of the „National Academy of Sciences”.
The Immunologist Prof. Dr. Michael Reth, Institute of Biology III, Faculty of Biology of the University of Freiburg has been elected as a new member of the “National Academy of Sciences” (NAS). By that decision, the NAS honours his studies on signal-transduction in B-Cells. With his research Michael Reth elucidated fundamental mechanisms of immune-cell-activation by the discovery of sub-units of the B-cell-receptor (BCR). The membership in the NAS is considered to be one of the highest awards within the scientific community. Michael Reth is the first professor of the University of Freiburg who received this award.
We sincerely congratulate Prof. Dr. Michael Reth for receiving this honour.
The full press release can be seen here. (in German)
After two excellent meetings in Rostock (2016) and Düsseldorf (2017) the 3rd Early Career Researcher Symposium on Cyanobacteria – short Cyano2018 – is going to take place in Freiburg from 12th – 14th of September 2018 and is organised by young researchers from the Wilde Lab and the Cyanolab (both part of the Institute of Biology 3)
This symposium was initiated by the “Vereinigung für Allgemeine und Angewandte Mikrobiologie” (VAAM) to offer a platform for scientific exchange and discussion to young researchers (master’s students, PhD students, young PostDocs) in the field of cyanobacteria.
Cyano2018 will include talks and poster presentations from early career researchers, as well as, keynote lectures by invited speakers. Registration will be open between April and 15th June 2018. You are welcome to register with an abstract for a talk or a poster presentation.
Please find the official Homepage of the Meeting here.
Please find the detailed news on www.cyanolab.de!
Institute of Biology 3
University of Freiburg
79104 Freiburg, Germany
DirectorProf. Dr. Wolfgang Hess
Vice-DirectorProf. Dr. Wolfgang Schamel
Administrative OfficerDr. Reinhard Gross
Prof. Dr. Ralf Baumeister
Prof. Dr. Ilka Diester
Prof. Dr. Wolfgang R. Hess
Prof. Dr. Maja Köhn
Prof. Dr. Thomas Laux
Prof. Dr. Carsten Mehring
Dr. Susana Minguet GarcÍa
Prof. Dr. Michael Reth
Prof. Dr. Stefan Rotter
Prof. Dr. Wolfgang Schamel
Dr. Ekkehard Schulze
Dr. Claudia Steglich
Prof. Dr. Annegret Wilde
Prof. Dr. Ad Aertsen
Prof. Dr. C. Bresch
Dr. P. Emschermann
Prof. Dr. F. Feix
Prof. Dr. Karl-Friedrich Fischbach
Prof. Dr. R. Hausmann
Prof. Dr. R. Hertel
Prof. Dr. K. Hilse
Dr. Gabor Igloi
Dr. W. Michalke
Dr. M. Neumann
Prof. Dr. Bodo Rak
Prof. Dr. Albrecht E. Sippel